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KMID : 0624620140470070399
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BMB Reports
2014 Volume.47 No. 7 p.399 ~ p.404
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Inhibition of mouse SP2/0 myeloma cell growth by the B7-H4 protein vaccine
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Mu Nan
Liu Nannan
Hao Qiang
Xu Yujin
Li Jialin
Li Weina
Wu Shouzhen
Zhang Cun
Su Haichuan
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Abstract
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B7-H4 is a member of B7 family of co-inhibitory molecules and B7-H4 protein is found to be overexpressed in many human cancers and which is usually associated with poor survival. In this study, we developed a therapeutic vaccine made from a fusion protein composed of a tetanus toxoid (TT) T-helper cell epitope and human B7-H4IgV domain (TT-rhB7-H4IgV). We investigated the anti-tumor effect of the TT-rhB7-H4IgV vaccine in BALB/c mice and SP2/0 myeloma growth was significantly suppressed in mice. The TT-rhB7-H4IgV vaccine induced high-titer specific antibodies in mice. Further, the antibodies induced by TT-rhB7-H4IgV vaccine were capable of depleting SP2/0 cells through complement-dependent cytotoxicity (CDC) in vitro. On the other hand, the poor cellular immune response was irrelevant to the therapeutic efficacy. These results indicate that the recombinant TT-rhB7-H4IgV vaccine might be a useful candidate of immunotherapy for the treatment of some tumors associated with abnormal expression of B7-H4.
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KEYWORD
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B7-H4, Tumor Escape, T-helper Epitope, Tetanus Toxoid, Tumor Vaccine
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